Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements

نویسندگان

چکیده

Promoters are upstream gene regulatory sequences recognized by transcription factors (TFs) involved in controlling initiation and progression. For modern crop improvement, the design of efficient constructs relies on promoter efficiency, tissue specificity, other characteristics that allow introgression agronomically relevant traits to overcome biotic abiotic stresses. Several constitutive viral promoters, such as pCaMV35S, remain widely employed transgenic plant generation, but their indiscriminate use leads silencing triggering metabolic penalties impacting fitness. The identification functional characterization plant-derived promoters can unveil alternatives commonly used non-homologous promoters; however, knowledge over them remains limited, especially for crops. This review summarizes drive foreign expression homologous heterologous systems, focusing inducible soybean from genes upregulated different Analyses these revealed 22 coincident cis-acting elements be synthetic engineering responsive multiple stresses and, therefore, efficiently expression, conferring desirable soybean. In addition, we also revisited commercial protected provide an update gain new insights into superior crops’ development.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Computational approaches to identify promoters and cis-regulatory elements in plant genomes.

The identification of promoters and their regulatory elements is one of the major challenges in bioinformatics and integrates comparative, structural, and functional genomics. Many different approaches have been developed to detect conserved motifs in a set of genes that are either coregulated or orthologous. However, although recent approaches seem promising, in general, unambiguous identifica...

متن کامل

Engineering of synthetic, stress-responsive yeast promoters

Advances in synthetic biology and our understanding of the rules of promoter architecture have led to the development of diverse synthetic constitutive and inducible promoters in eukaryotes and prokaryotes. However, the design of promoters inducible by specific endogenous or environmental conditions is still rarely undertaken. In this study, we engineered and characterized a set of strong, synt...

متن کامل

Computational discovery of soybean promoter cis-regulatory elements for the construction of soybean cyst nematode-inducible synthetic promoters.

Computational methods offer great hope but limited accuracy in the prediction of functional cis-regulatory elements; improvements are needed to enable synthetic promoter design. We applied an ensemble strategy for de novo soybean cyst nematode (SCN)-inducible motif discovery among promoters of 18 co-expressed soybean genes that were selected from six reported microarray studies involving a comp...

متن کامل

DBTGR: a database of tunicate promoters and their regulatory elements

The high similarity of tunicates and vertebrates during their development coupled with the transparency of tunicate larvae, their well-studied cell lineages and the availability of simple and efficient transgenesis methods makes of this subphylum an ideal system for the investigation of vertebrate physiological and developmental processes. Recently, the sequencing of two different Ciona genomes...

متن کامل

Signal integration on plant promoters

Gene promoters perceive numerous signals and integrate this information into a single response, the transcriptional activity of a gene. It was speculated that covalent modification of histones on the promoters might have an important function in storage and integration of signals. Using the genes for the core proteins of C4 metabolism in maize as a model, we associated the perception of specifi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biotechnology Research and Innovation

سال: 2021

ISSN: ['2452-0721']

DOI: https://doi.org/10.4322/biori.202105